EFFECT OF DIFFERENT LED WAVELENGTHS ON SURVEILLANCE CURVE OF ESCHERICHIA COLI AB1157 AND BW9091 WITH POSSIBLE GENOTOXIC ACTIONS
Keywords:
LED, Escherichia coli, DNA, surveillance, healingAbstract
Physiotherapy plays a function in the facilitation of repair process, once it utilized resources as laser and ultrasound therapy, sonic waves, microwaves and the phototherapy for processing the modulating repair. The utilization of the LED (Light Emitting Diode) in the wound healing process is based on its effects antibacterial, anti-inflammatory and cicatrization. The objective of this work is to investigate the effects of the LED of different wavelengths on surveillance of one cellular type proficient in repair DNA damage. It was used Escherichia coli (E.coli) strains proficient in repair DNA damage (AB1157) or not proficient (BW9091). Exponentially E. coli AB1157 or BW9091 cultures were incubated at liquid rich medium overnight. Aliquots were spread onto Petri dishes containing solidified rich medium and irradiated with LED of different wavelengths, the colonies units were counted after overnight and the survival fraction was calculated. The results show cytotoxic effect of blue LED on E. coli AB1157, and of the green and blue LEDs on E.coli BW9091. In conclusion, the results of bacterial surveillance showed cytotoxic effect manly for blue light (bacterial effect) and green light (anti-inflammatory effect).
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